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Version française > Savoir faire > PlatePlateau "Chimie analytique et de synthèse des lipides"

Equipments

Lipid chemistry - analysis and synthesis: Gas Chromatography, High Performance Liquid Chromatography, High Performance Thin Layer Chromatography-densitometry system, Thin Layer Chromatography-mass, Puriflash interchim Chromatography.

 

Cell and tissue culture: hypoxia workstation, human breast cancer (MCF-7, MCF-7dox, MDA-MB-231, MDA-MB-468, MDA-MB-435s…), human lung cancer (H460, H23, A549, Calu-1, Calu-3…), human prostate cancer (PC3, LnCaP…), human colon cancer (HCT116, SW48…), murine mammary cancer (4T1, EMT-6) cell lines, non-cancer cell lines and primary cultures (HepaRG, MCF-10A, BEAS-2B, LCHAD, fibroblasts, …), human prostate cancer organotypic cultures from tumor explants, cell culture models derived from biopsy samples of breast, colon and prostate cancer patients.

 

Molecular and cell biology: AktaPure FPLC system (chromatography system for fast purification of proteins), XCelligence (Real-Time Cell Analysis systems for monitoring of cell proliferation, cell size/morphology, cell adhesion, and cell invasiveness/migration), PhastSystem (fast separation of proteins by native or SDS-PAGE).

 

Microscopy: time–lapse epifluorescence microscopy, epifluorescence imaging.

 

Fluorescence analyses: spectrofluorimeters, fluorescence imaging workstation (Flexstation).

 

Mitochondrial bioenergetics: oxygraph [high resolution (2) and conventional (1)], luminometer (1).

 

Electrophysiology: conventional patch-clamp systems equipped with epifluorescence imaging (3), automated patch-clamp platform (Nanion patchliner).

 

Tumor and adipose tissues libraries: human, rat and mouse cancer tissues (mammary, colon, prostate) and non-cancer associated adipose tissues.

 

Anatomopathology and Immunohistochemistry: automated immunohistochemistry labeling system (Benchmark XT), manual tissue arrayer system (BeecherInstruments), staining system, microscopy, automated glass coverslipper system (Tissue-Tek), automated microtome system (Thermo Electron), cryotomy system (Thermo Electron).


Mise à jour de la page 07/11/2016

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